Ecio vowed to take revenge by releasing the Romans

Desmond and his allies have retreated to Monteriđioni villa, and Desmond enters the Animus again to continue Eciova memories, especially to find a location Apples. After meeting with Rodrigo Borgia, Ezio returned to Monteriđioni but were soon attacked the villa forces were under the command of Cesare Borgia, Rodrigo's son. Mario was killed and apple is stolen Guilty Gear Costume
. Ecio vowed to take revenge by releasing the Romans. While Ezio works undercover at turning the city against Borgia, gains followers who want to join his case, and he trains them to Asasinski way. In an attempt to assassinate Cesare and Rodrigo in the trap Sant'Angelo, Ecio testify Caesar which forced his father to eat a poisoned apple by Rodrigo prepared to kill his son. Ezio rushes Caesar and eventually traps and takes the apple back. Cesare was taken to the prison in Viana in Spain, but he managed to escape with the help of its allies. Assassins are battling against Caesar and other loyal troops Borgia. Finally Ezio throwing Cesare with the castle walls and kill him. No more threats on the horizon, Ezio takes the apple and sakrva beneath the Coliseum. At present, Desmond managed to pass through the underground chambers beneath the Colosseum to locate Apple. As he picked it up, Desmond testifies another holographic figure, calling itself Juno and which controls Desmond to stab Lucy (Lucy because she fled Templars) before he fell into a coma.

Brotherhood shares many of the characteristics of the last game, albeit taking place primarily in Rome. Similar to the villa, the player can spend money to buy and improve stores and other institutions during the entire city in order to increase the revenue that can be expensive than that; However, of the players will be required to destroy Bordžijine towers that control various parts of the city before it can do this. Introduced the Brotherhood of Assassins, which, after a rescue of the citizens of some events, players can invite the public to join the Assassins; They can be sent to a remote location through Europe to collect experience and money, or they may be called to help the player directly to the mission Cosplay Australia
. For the first time in the franchise, the game introduces online multiplayer. Players may play as an employee of Abstergo, which, through the use of the Animus, surviving memories of the Renaissance Templar in different modes to play.

Desmond becomes aware of in the virtual zone in the Animus, Animus Island, where he meets digital personality of the Subject 16 Subject 16 explained that the trauma suffered by the stabbing of the Lusi, Desmond must go through the memories of Altair and ECIA that could separate his mind from their i to be able to wake up from a coma; However, it would destabilize the island while Animus returns segments of memory and at one point entity 16 sacrificed themselves to Desmond could not continue. Desmond returns to Eciovu story many years after the Brotherhood-a, where Ezio wants to know about the origin of the Assassins and travel to Masyaf to find their first fort. Finds Altairovu library, it is however locked with five keys that the Templars also seek, believing that therein lies a great power Hiiro no Kakera Costume
. Ezio travels to Constantinople during the Ottoman era where it is said that Nicolo Polo hid the keys and finds a city agitated quarreling brothers Selim and Ahmet vying for the title of Sultana, backed by the Templars. Searching for keys, Ecio luck and falls in love with Sofia Sartor. Ahmet was eventually identified as a Templar agent, killing him and Selim ECIU thanks for the help, but it leaches out of the city for the sake of their own good.